Repository: UIHistories Project: Board of Trustees Minutes - 1880 [PAGE 124]

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122 . 1. The number of c.c. it took to precipitate copper solution: total number of c.c. : : .05 (grains of grape sugar required to precipitate 10 c.c. of Fehling's solution) : x. X multiplied by .95 will give the grains of sugar in 10 grains of seed. The residue, B, was washed on the filter with alcohol acidulated with sulphuric acid and finally with water, to dissolve the gluten. Then the residue was washed off the filter into a beaker diluted to about 400 c.c. 5 c.c. of sulphuric acid added, and the whole boiled on a water bath until the liquid had no milky appearance. It was then filtered through an equipoised filter and washed. Solution==C. Kesidue=D. Solution C was diluted to 500 c.c. 50 c.c. of this solution were transferred to a prescription bottle and then treated as above for sugar and dextrine. From the grape sugar obtained, the amount of starch was obtained. Eesidue D was boiled with hot sodium hydroxide, again thrown upon the filter and washed with the same solvent; afterwards, with hot water, then with alcohol, and finally with ether. The washed residue was dried at 110° C , weighed, ignited, and the amount of ash deducted. The remainder was estimated as fiber. Water.—For the estimation of water, the ground seed was weighed in a glass-stoppered test tube. After weighing, the glass stopper was replaced by a rubber one, through which passed two glass tubes, bent at right 'angles. One of these tubes was connected with an aspirator; the other, with a calcium chloride tube and a sulphuric acid drying bottle. The test tube and its contents were then placed in an opening of a drying oven, whose temperature was between 100 and 110° C. During the operation, a current of air, passing through the sulphuric acid and calcium chloride tube, thus drying it, was drawn into the tube and the moisture sucked out by means of the aspirator. When the weight became constant, the loss was estimated as water. Ash.—The contents of the tube were transferred to a platinum crucible, incinerated, and ash weighed. Albuminoids.—One grain of the ground seed was ignited with soda lime. The substance was intimately mixed with a portion of soda lime sufficient to fill a 14-inch combustion tube two-thirds full. About two inches of the tube were filled with soda lime, then the mixture of soda lime and substance added, the mortar rinsed with soda lime, and finally the rinsings and enough soda lime added to nearly fill the tube. A plug of asbestos was put in, and the tube gently tapped to insure an air passage throughout its length. Will's bulbs were charged with a deci-normal solution of oxalic acid. The tube being placed in the combustion furnace was connected with the bulbs. The fore part of the tube, containing the soda lime only, was heated to redness, then heat applied, one jet at a time, along the entire length of the tube, care being taken that the combustion was completed in that portion of the tube where heat was applied before other jets were turned on, and also that the combustion was not too rapid. After the combustion was